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Performance of FACS (Fluorescence-Activated Cell Sorter) instruments at Institute of Molecular Biology, Academia Sinica, Taipei, TAIWAN

Fluorescence detection

FITC (AF488) channel

PE channel

APC (AF647) channel

Electronic cell sorting

Exp 1: 3000 events per second (2-way sorting to the same side)

Exp 2: 3000 events per second (2-way sorting to the same side)

Exp 3: 8000 events per second (2-way sorting to the same side)

Exp 4: 8000 events per second (1-way sorting)

Exp 5: 8000 events per second (2-way sorting to different sides)

Exp 6: 8000 events per second (4-way sorting, 2 subsets each to different sides, on BD Aria SORP)

Exp 7: 8000 events per second (4-way sorting, 2 subsets each to different sides, on Beckman Astrios EQ)

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